Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Identification of a candidate tumor-suppressor gene specifically activated during Ras-induced senescence
Δημιουργός/Συγγραφέας:	Barradas, M. [EL] Γκόνος, Ευστάθιος[EN] Gonos, Efstathios S. Zebedee, Z. Kolettas, E. Petropoulou, C. Delgado, M. D. Leon, J. Hara, E. Serrano, M.
Εκδότης:	Academic Press Incorporated Elsevier Science
Ημερομηνία:	2002
Γλώσσα:	Αγγλικά
ISSN:	0014-4827
DOI:	10.1006/excr.2001.5434
Περίληψη:	Normal cells display protective responses against oncogenes. Notably, oncogenic Ras triggers an irreversible proliferation arrest that is reminiscent of replicative senescence and that is considered a relevant tumor-suppressor mechanism. Here, we have used microarrayed filters to identify genes specifically upregulated in Ras-senescent human fibroblasts. Among the initial set of genes selected from the microarrays, we found the cell-cycle inhibitor p21Cip1/Waf1, thus validating the potency of the screening to identify markers and mediators of Ras-senescence. A group of six genes, formed by those more highly upregulated during Ras-senescence, was analyzed in further detail to evaluate their specificity. In particular, we examined their expression in cells overexpressing Ras but rendered resistant to Ras-senescence by the viral oncoprotein E1a; also, we have studied their expression during replicative senescence, organismal aging, H2O2-induced senescence, and DNA damage. In this manner, we have identified a novel gene, RIS1 (for Ras-induced senescence 1), which is not upregulated in association to any of the above-mentioned processes, but exclusively during Ras-senescence. Furthermore, RIS1 is also upregulated by the transcriptional factor Ets2, which is a known mediator of Ras-induced senescence. Interestingly, RIS1 is located at chromosomal position 3p21.3 and, more specifically, it is included in a short segment of just 1 Mb previously defined by other investigators for its tumor-suppressor activity. In summary, we report the identification of a novel gene, RIS1, as a highly specific marker of Ras-induced senescence and a candidate tumor-suppressor gene.
Τίτλος πηγής δημοσίευσης:	Experimental Cell Research
Τόμος/Κεφάλαιο:	273
Τεύχος:	2
Σελίδες:	127-137
Θεματική Κατηγορία:	[EL] Κυτταρολογία[EN] Cytology [EL] Μοριακή Βιολογία[EN] Molecular Biology [EL] Νεοπλάσματα. Όγκοι. Ογκολογία (περ. Καρκίνος, κακινογόνες ουσίες)[EN] Neoplasms. Tumors. Oncology (Incl.cancer, carcinogens)
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://doi.org/10.1006/excr.2001.5434
ΙΒΦΧΒ: αρχειακή συλλογή:	Ινστιτούτο Βιολογικών Ερευνών και Βιοτεχνολογίας (ΙΒΕΒ) (έως 2012)
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο