Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Pleurotus eryngii Mushrooms Fermented with Human Fecal Microbiota Protect Intestinal Barrier Integrity: Immune Modulation and Signalling Pathways Counter Deoxycholic Acid-Induced Disruption in Healthy Colonic Tissue
Δημιουργός/Συγγραφέας:	Kerezoudi, Evangelia N Zervakis, Georgios I [EL] Πλέτσα, Βασιλική[EN] Pletsa, Vassiliki Kyriacou, Adamantini Brummer, Robert J Rangel, Ignacio
Ημερομηνία:	2025-02-14
Γλώσσα:	Αγγλικά
ISSN:	2072-6643
DOI:	10.3390/nu17040694
Άλλο:	40005021
Περίληψη:	Background: This study explores the potential of the Pleurotus eryngii mushroom fermentation supernatant (FS-PEWS) as an intervention for mitigating sodium deoxycholate (SDC)-induced intestinal barrier dysfunction and inflammation. Methods: FS-PEWS was assessed for its protective effects against SDC-induced barrier dysfunction and inflammation using an in vitro Caco-2 cell model and ex vivo colonic biopsies from healthy adult donors, where barrier integrity, permeability, immunomodulation and receptor-mediated pathways were evaluated. Results: In Caco-2 cells, SDC exposure downregulated ZO-1, occludin, and claudin-1 expression, with FS-PEWS restoring ZO-1 and claudin-1 levels while maintaining cell viability. In colonic biopsies from healthy adults, FS-PEWS maintained tissue integrity and selectively mitigated transcellular permeability without affecting paracellular permeability when combined with the stressor. Additionally, FS-PEWS exhibited potent anti-inflammatory effects, reducing pro-inflammatory cytokines, e.g., TNF-α, IL-6, and IL-1β and modulating receptor-mediated pathways, i.e., TLR-4, dectin-1. Conclusions: These results demonstrate the potential of FS-PEWS to sustain intestinal barrier function and modulate immune responses under stress, highlighting its therapeutic potential for managing gut barrier dysfunction and inflammation associated with microbial metabolite-induced disruptions.
Τίτλος πηγής δημοσίευσης:	Nutrients
Τόμος/Κεφάλαιο:	17
Τεύχος:	4
Θεματική Κατηγορία:	[EL] Χημική Βιολογία[EN] Chemical Biology [EL] Ανοσολογία[EN] Immunology [EL] Βιοχημεία[EN] Biochemistry [EL] Ιατρική (Γενικά)[EN] Medicine (General) [EL] Γεωργία[EN] Agriculture
Λέξεις-Κλειδιά:	Pleurotus eryngii mushrooms Ussing chamber cytokines deoxycholic bile acid gut barrier signalling pathway
Χρηματοδότης:	European Union Greece
Χρηματοδοτικό πρόγραμμα:	Competitiveness, Entrepreneurship and Innovation
Αναγνωριστικό χρηματοδοτικού προγράμματος:	T1EDK-03404
Κάτοχος πνευματικών δικαιωμάτων:	: © 2025 by the authors. Licensee MDPI, Basel, Switzerland.
Όροι και προϋποθέσεις δικαιωμάτων:	This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/ licenses/by/4.0/).
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://doi.org/10.3390/nu17040694
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο