Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Effects of In Vitro Fermented Pleurotus eryngii on Intestinal Barrier Integrity and Immunomodulation in a Lipopolysaccharide-Induced Colonic Model
Δημιουργός/Συγγραφέας:	Kerezoudi, Evangelia N Saxami, Georgia Zervakis, Georgios I [EL] Πλέτσα, Βασιλική[EN] Pletsa, Vassiliki Brummer, Robert J Kyriacou, Adamantini Rangel, Ignacio
Ημερομηνία:	2025-02-11
Γλώσσα:	Αγγλικά
ISSN:	2227-9059
DOI:	10.3390/biomedicines13020430
Άλλο:	40002843
Περίληψη:	Background: This study investigates the impact of fermentation supernatants (FSs) from Pleurotus eryngii whole mushrooms (PEWS), as well as its subcomponents, digested (PEWSD) and extracted (PEWSE) forms, on intestinal barrier function and immune modulation in lipopolysaccharide (LPS) -stimulated Caco-2 cells. Methods: Gene expression of tight junction (TJs) genes, cytokines, and key immune/metabolic receptors was assessed via qRT-PCR, while cytokine protein levels were measured using ELISA to explore post-transcriptional regulation. Results: LPS challenge significantly downregulated TJs zonula occludens-1 (ZO-1,) occludin, and claudin-1, compromising epithelial integrity. Treatment with FS-PEWS notably restored ZO-1 and occludin expression, outperforming FS-PEWSD and FS-PEWSE, which only partially mitigated the LPS-induced damage. FS-PEWS further demonstrated potent immunomodulatory effects, upregulating anti-inflammatory IL-10 and pro-inflammatory cytokines such as IL-8 and TNF-α. The activation of key receptors like TLR-2 and mTOR suggests that FS-PEWS modulates critical immune and metabolic pathways, such as NF-kB signaling, to maintain immune homeostasis. Although mRNA expression of pro-inflammatory cytokines was altered, no corresponding protein release was detected, suggesting potential post-transcriptional regulation. Conclusions: FS-PEWS preserves intestinal barrier integrity and modulates immune responses, particularly in low-grade inflammation, highlighting the whole food matrix's role in enhancing its bioactivity and functional food potential.
Τίτλος πηγής δημοσίευσης:	Biomedicines
Τόμος/Κεφάλαιο:	13
Τεύχος:	2
Θεματική Κατηγορία:	[EL] Χημική Βιολογία[EN] Chemical Biology [EL] Μοριακή Βιολογία[EN] Molecular Biology [EL] Ανοσολογία[EN] Immunology [EL] Γεωργία[EN] Agriculture
Λέξεις-Κλειδιά:	Pleurotus eryngii gut barrier function immune response lipopolysaccharides tight junctions
Χρηματοδότης:	European Union Greece
Χρηματοδοτικό πρόγραμμα:	Competitiveness, Entrepreneurship & Innovation
Αναγνωριστικό χρηματοδοτικού προγράμματος:	T1EDK-03404
Κάτοχος πνευματικών δικαιωμάτων:	© 2025 by the authors. Licensee MDPI, Basel, Switzerland.
Όροι και προϋποθέσεις δικαιωμάτων:	This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/ licenses/by/4.0/).
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://doi.org/10.3390/biomedicines13020430
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο