Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Accessing new dimensions in high-resolution materials exploration by cryo-electron microscopy
Δημιουργός/Συγγραφέας:	Koralli, Panagiota Kyrilis Fotis Hamdi, Farzad [EL] Χώχος, Χρήστος[EN] Chochos, Christos [EL] Καστρίτης, Παναγιώτης[EN] Kastritis, Panagiotis
Ημερομηνία:	2026
Γλώσσα:	Αγγλικά
ISSN:	0927796X
DOI:	10.1016/j.mser.2026.101192
Περίληψη:	AbstractCryo-electron microscopy (cryo-EM) is employed for structural analyses, visualizing high-resolution information across scales, i.e., from tissues to small molecules. Developments allowing near-atomic resolution cryo-EM imaging of biological macromolecules were recognized by the 2017 Nobel Prize in Chemistry. In the materials science domain, despite specific applications, cryo-EM analysis presents discrete challenges related to sample preparation, imaging, and data interpretation, as function of the sample’s innate physical chemistry. Here, we review recent progress in the field, focusing on overcoming intricacies in analysis of soft matter (e.g., polymers, gels, colloids) and functional materials like metal-organic frameworks (MOFs), covalent organic frameworks (COFs), and hybrid perovskites. Sample preparation, including grid selection, surface treatments, and vitrification methods are compared, highlighting their effects on image quality and artifact reduction. Advanced cryo-EM methods, and their combination with scanning transmission electron microscopy (STEM) and low-dose energy loss spectroscopy (EELS) are also examined to evaluate their potential in describing complex molecular structures and their conformational heterogeneity. This review, overall, highlights the need for standardized, statistically empowered cryo-EM protocols inspired from biological applications, and integration of emerging technologies like machine learning and open data initiatives, to ultimately incorporate cryo-EM into materials research as a fundamental method.
Τίτλος πηγής δημοσίευσης:	Materials Science and Engineering: R: Reports
Τόμος/Κεφάλαιο:	169
Θεματική Κατηγορία:	[EL] Νανοτεχνολογία[EN] Nanotechnology [EL] Φασματοσκοπία[EN] Spectroscopy [EL] Βιοιατρική μηχανική. Ηλεκτρονική. Ενοργάνιση[EN] Biomedical engineering. Electronics. Instrumentation [EL] Ηλεκτρική μηχανική. Ηλεκτρονική. Πυρινική μηχανικη[EN] Electrical engineering. Electronics. Nuclear engineering [EL] Χημική μηχανική[EN] Chemical engineering
Λέξεις-Κλειδιά:	Advanced imaging techniques Cryogenic electron microscopy Material characterization Materials science Soft materials
Χρηματοδοτικό πρόγραμμα:	Horizon Europe ERA Chair “hot4cryo” ZIK HALOmem RTG 2467 CRC 1664 2nd Call for H.F.R.I. Research Projects to Support Faculty Members & Researchers Basic Research Financing (Horizontal support of all Sciences)
Αναγνωριστικό χρηματοδοτικού προγράμματος:	101086665 03Z22HN23 03Z22HI2 03COV04 ZS/2016/04/78115 ZS/2024/05/187255 391498659 514901783 4694 17007
Κάτοχος πνευματικών δικαιωμάτων:	© 2026 The Author(s). Published by Elsevier B.V.
Όροι και προϋποθέσεις δικαιωμάτων:	This is an open access article under the CC BY license. http://creativecommons.org/licenses/by/4.0/
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://doi.org/10.1016/j.mser.2026.101192
Σημειώσεις:	This work was supported by the Horizon Europe ERA Chair \u201Chot4cryo\u201D, project number 101086665 (to P.L.K.), the Federal Ministry for Education and Research (BMBF ZIK HALOmem, grant nos. 03Z22HN23 , 03Z22HI2 and 03COV04 (to P.L.K.), the European Regional Development Funds ( EFRE ) for Saxony-Anhalt (grant no. ZS/2016/04/78115 and grant no. ZS/2024/05/187255 (to P.L.K.), the German Research Foundation (DFG, project numbers 391498659, RTG 2467 and 514901783 , CRC 1664), the Martin Luther University Halle-Wittenberg and the National Hellenic Research Foundation. C.L.C. acknowledges the Hellenic Foundation for Research and Innovation (H.F.R.I.) under the \u201C2nd Call for H.F.R.I. Research Projects to support Faculty Members & Researchers\u201D (Project Number: 4694) and under the \u201CBasic research Financing (Horizontal support of all Sciences)\u201D call under the National Recovery and Resilience Plan \u201CGreece 2.0\u201D funded by the European Union \u2013Next Generation EU (H.F.R.I. Project Number: 17007 ). We would like to thank all the Chochos and Kastritis lab members for fruitful discussions.
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο