Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Microfluidic assembly and biomimetic lipid coating modulate the structure, stability, and biological interactions of P(DMAEMA-co-SMA)/DNA lipopolyplexes
Δημιουργός/Συγγραφέας:	Tsichlis, Ioannis Vardaxi, Antiopi Gomez, Timothy Athanasaki, Antonia Forys, Alexander Trzebicka, Barbara Richardson, Simon C W Chrissopoulou, Kiriaki Anastasiadis, Spiros H [EL] Πίσπας, Στέργιος[EN] Pispas, Stergios Douroumis, Dennis Demetzos, Costas
Ημερομηνία:	2026-02
Γλώσσα:	Αγγλικά
ISSN:	01418130
DOI:	10.1016/j.ijbiomac.2026.150678
Άλλο:	41638267
Περίληψη:	In this study, we present the development and characterization of biomimetic lipopolyplexes using the pH-responsive cationic copolymer, P(DMAEMA-co-SMA), DNA, and membrane-mimicking lipids. The copolymer was synthesized via RAFT polymerization and characterized by size exclusion chromatography, 1H NMR spectroscopy, ATR-FTIR spectroscopy, and acid-base titration for proton buffering capacity. A custom-designed 3D-printed microfluidic chip with embedded microstructures was utilized to form polyplexes under controlled flow conditions, followed by a post lipid-coating step via lipid film hydration. The statistical copolymer P(DMAEMA-co-SMA) was utilized to condense DNA 50 bp at various nitrogen-to-phosphate (N/P) ratios, yielding polyplexes with distinct physicochemical characteristics. Lipid coating of preformed polyplexes enhanced colloidal stability under storage and biorelevant conditions, highlighting its critical role in maintaining nanoparticle integrity. Cryo-TEM analysis revealed the coexistence of multiple nanostructures with small-angle X-ray scattering (SAXS) supporting these findings and demonstrating pH-dependent organization that provides insights into their structural behavior under biologically relevant conditions. In vitro cytotoxicity and hemocompatibility assays indicated that the developed P(DMAEMA-co-SMA)/DNA lipopolyplexes are well tolerated compared to polyethylenimine (PEI), the gold standard in non-viral gene delivery. Confocal microscopy showed enhanced cellular uptake, endosomal escape, and cytoplasmic distribution in HeLa cells, supporting the potential of the prepared nanocomplexes for efficient intracellular gene delivery. Overall, this study presents P(DMAEMA-co-SMA)/DNA lipopolyplexes as a stable, biocompatible, and effective gene delivery platform and demonstrates how biomimetic lipid coating can modulate the stability and biological interactions of DNA nanocomplexes.
Τίτλος πηγής δημοσίευσης:	International journal of biological macromolecules
Τόμος/Κεφάλαιο:	346
Θεματική Κατηγορία:	[EL] Νανοτεχνολογία[EN] Nanotechnology [EL] Κυτταρολογία[EN] Cytology
Λέξεις-Κλειδιά:	DNA Lipopolyplexes Microfluidic assembly pH-responsive cationic copolymer Biomimetic lipid coating Cytotoxicity Cellular uptake
Χρηματοδότης:	State Scholarships Foundation (IKY) British Embassy Athens British Council Greece Uni-Pharma Kleon Tsetis Pharmaceutical Laboratories S.A. Hellenic Foundation for Research and Innovation (HFRI)
Χρηματοδοτικό πρόγραμμα:	SAXS-SOFT
Αναγνωριστικό χρηματοδοτικού προγράμματος:	2nd Call for Greek-British Cooperation on Short-Term PhD Scholarships SAXS-SOFT
Κάτοχος πνευματικών δικαιωμάτων:	© 2026 The Authors. Published by Elsevier B.V.
Όροι και προϋποθέσεις δικαιωμάτων:	This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://doi.org/10.1016/j.ijbiomac.2026.150678
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο