Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Metabolomics and proteomics insights into hepatic responses of weaned piglets to dietary Spirulina inclusion and lysozyme supplementation
Δημιουργός/Συγγραφέας:	Martins, Cátia Falcão [EL] Ματζαπετάκης, Μανόλης[EN] Matzapetakis, Manolis Ribeiro, David M Kuleš, Josipa Horvatić, Anita Guillemin, Nicholas Eckersall, Peter David Freire, João P B Almeida, André M Prates, José A M
Ημερομηνία:	2024-11-06
Γλώσσα:	Αγγλικά
ISSN:	1746-6148
DOI:	10.1186/s12917-024-04339-7
Άλλο:	39506864
Περίληψη:	Studying the effect of dietary Spirulina and lysozyme supplementation on the metabolome and proteome of liver tissue contributes to understanding potential hepatic adaptations of piglets to these novel diets. This study aimed to understand the influence of including 10% Spirulina on the metabolome and proteome of piglet liver tissue. Three groups of 10 post-weaned piglets, housed in pairs, were fed for 28 days with one of three experimental diets: a cereal and soybean meal-based diet (Control), a base diet with 10% Spirulina (SP), and an SP diet supplemented with 0.01% lysozyme (SP + L). At the end of the trial, animals were sacrificed and liver tissue was collected. Metabolomics analysis (n = 10) was performed using NMR data analysed with PCA and PLS-DA. Proteomics analysis (n = 5) was conducted using a filter aided sample preparation (FASP) protocol and Tandem Mass Tag (TMT)-based quantitative approach with an Orbitrap mass spectrometer.
Τίτλος πηγής δημοσίευσης:	BMC veterinary research
Τόμος/Κεφάλαιο:	20
Τεύχος:	1
Θεματική Κατηγορία:	[EL] Βιοχημεία[EN] Biochemistry [EL] Φασματοσκοπία[EN] Spectroscopy [EL] Βιοτεχνολογία[EN] Biotechnology [EL] Φυσιολογία[EN] Physiology
Λέξεις-Κλειδιά:	Carbohydrase Liver metabolome Liver proteome Lysozyme Piglets Spirulina
Χρηματοδότης:	Fundação para a Ciência e a Tecnologia European Commission
Αναγνωριστικό χρηματοδοτικού προγράμματος:	PTDC/CVT-NUT/5931/2014 UIDB/00276/2020 (CIISA) LA/P/0059/2020 (AL4AnimalS) UIDB/04129/2020 (LEAF) FP7 “VetMedZg” Project, grant number 621394 Portugal 2020 grant (08/SI/3399/2015) SFRH/BD/143992/2019
Κάτοχος πνευματικών δικαιωμάτων:	© The Author(s) 2024
Όροι και προϋποθέσεις δικαιωμάτων:	Open Access. This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://doi.org/10.1186/s12917-024-04339-7
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο