Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Viscoelastic profile of exopolysaccharides produced from Schizophyllum commune in bioreactor cultures utilizing brewer's spent grain: A sustainable bioprocess
Δημιουργός/Συγγραφέας:	Pilafidis, Sotiriοs Tsouko, Erminta Diamantopoulou, Panagiota Gkatzionis, Konstantinos Sarris, Dimitris [EL] Παπαγιαννόπουλος, Αριστείδης[EN] Papagiannopoulos, Aristeidis
Ημερομηνία:	2025
Γλώσσα:	Αγγλικά
ISSN:	01418130
DOI:	10.1016/j.ijbiomac.2025.147004
Περίληψη:	This study explores the potential valorization of brewer's spent grain (BSG) by Schizophyllum commune as an alternative low-cost and sustainable feedstock to produce fungal biomass rich in bioactive compounds and exopolysaccharides (EPS). Submerged cultures were performed in a 6 l bioreactor achieving maximum values of 9.8 g/l biomass and 7.3 g/l EPS with BSG as the substrate. Compared with traditional glucose-based culture media, BSG led to a 1.9-fold increase in biomass and a 2.3-fold increase in EPS production. The biomass produced was rich in protein (21.4 % w/w d.w) and β-glucans (44.6 % w/w d.w). The β-glucans content in the EPS was 44.1 %, however, it was much lower for α-glucans (0.5 %). 1H NMR, FTIR and Raman spectrometry revealed the characteristic peaks of β-glucans and TGA analysis showed the high thermal stability of the EPS. Particle tracking microrheology and viscometry showed that the produced EPS displayed power-law viscoelasticity and shear-thinning behavior respectively. These characteristics became stronger as concentration increased. The gel strength and elasticity were stronger for the EPS from BSG. This study represents the first comprehensive report of using unrefined brewer's spent grain as the sole carbon source for S. commune EPS production with detailed viscoelastic characterization, establishing a circular bioeconomy approach that advances the potential of fungal biopolymers for industrial biotechnology.
Τίτλος πηγής δημοσίευσης:	International Journal of Biological Macromolecules
Τόμος/Κεφάλαιο:	323
Θεματική Κατηγορία:	[EL] Βιοτεχνολογία[EN] Biotechnology [EL] Βιοχημεία[EN] Biochemistry
Λέξεις-Κλειδιά:	Bioeconomy Biopolymers Circular Exopolysaccharide Fungi Glucan Rheology
Κάτοχος πνευματικών δικαιωμάτων:	© 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://doi.org/10.1016/j.ijbiomac.2025.147004
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο