Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Stimuli-responsive laccase/chitosan-g-PNIPAM complexes: A sustainable strategy for biodegradation of organic pollutants
Δημιουργός/Συγγραφέας:	Petrila, Larisa-Maria Karayianni, Maria Vasiliu, Tudor Puf, Răzvan Mihai, Marcela [EL] Πίσπας, Στέργιος[EN] Pispas, Stergios
Ημερομηνία:	2025-08-11
Γλώσσα:	Αγγλικά
ISSN:	01418130
DOI:	10.1016/j.ijbiomac.2025.146754
Άλλο:	40803466
Περίληψη:	Hybrid nanostructures from synthetic and biological macromolecules emerged as a significant research topic as they can find applications in biomedicine, catalysis, and environmental remediation. Herein, innovative nanostructures in the form of electrostatic complexes were prepared by the interaction of laccase with a hybrid graft copolymer, CHI-g-PNIPAM, obtained by grafting poly(N-isopropylacrylamide) containing carboxylate end-groups onto chitosan chains. The size, charge, morphology, and stability of the obtained nanostructures were investigated through light scattering measurements and scanning transmission electron microscopy, demonstrating the effect of the ratio between the components on the properties of the obtained co-assembled nanostructures. Also, molecular dynamics simulations were performed to determine the interaction mechanism between the copolymer and the enzyme. The use of the copolymer imparted the nanostructures with improved stability in various conditions and thermo-responsive behaviour, while the incorporation of laccase into such nanostructures enhanced the catalytic activity of the enzyme. The obtained hybrid nanostructures were successfully employed as catalysts for the degradation of indigo carmine and Congo red from aqueous solutions, demonstrating their potential as novel catalysts for cutting-edge applications.
Τίτλος πηγής δημοσίευσης:	International journal of biological macromolecules
Τόμος/Κεφάλαιο:	322
Τεύχος:	Pt 2
Θεματική Κατηγορία:	[EL] Νανοτεχνολογία[EN] Nanotechnology [EL] Βιοχημεία[EN] Biochemistry [EL] Βιοτεχνολογία[EN] Biotechnology
Λέξεις-Κλειδιά:	Chitosan Dye degradation Enzymatic catalysis Hybrid nanostructures Poly(N-isopropylacrylamide) Trametes versicolor laccase
Χρηματοδότης:	Romanian Authority for Research European Union
Χρηματοδοτικό πρόγραμμα:	Horizon Europe research and innovation program
Αναγνωριστικό χρηματοδοτικού προγράμματος:	PNRR-III-C9-2022-I8-201 101086667
Κάτοχος πνευματικών δικαιωμάτων:	Copyright © 2025 The Authors. Published by Elsevier B.V. All rights reserved.
Όροι και προϋποθέσεις δικαιωμάτων:	This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://doi.org/10.1016/j.ijbiomac.2025.146754
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο