Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	A Study on the Structure, Optical Properties and Cellular Localization of Novel 1,3-Benzothiazole-Substituted BODIPYs
Δημιουργός/Συγγραφέας:	Kirkilessi, Olga Arapatzi, Christina [EL] Ραϊς, Χεριμπέρτ[EN] Reis, Heribert Kostourou, Vassiliki [EL] Προύσης, Κυριάκος[EN] Prousis, Kyriakos [EL] Καλογεροπούλου, Θεοδώρα[EN] Calogeropoulou, Theodora
Ημερομηνία:	2023
Γλώσσα:	Αγγλικά
ISSN:	2079-6447
DOI:	10.3390/colorants3010002
Περίληψη:	Abstract A library of seven novel 1,3-benzothiazole-substituted BODIPY derivatives with tunable optical properties was synthesized. The new fluorescent dyes exhibited bathochromically shifted absorptions (up to 670 nm) and emissions centered in the red and near-infrared spectral region (up to 677 nm) in comparison to the parent compound 8-phenyl BODIPY (λabs: 499 nm, λemi: 508 nm). (TD)DFT calculations were performed to rationalize the spectroscopic properties of the new dyes. The cellular biodistribution of the new BODIPY dyes, their fluorescence stability and toxicity were investigated in both living and fixed fibroblasts using time-lapse fluorescent imaging and confocal microscopy. Six of the seven new dyes were photostable and non-toxic in vitro at 10 μM concentration. In addition, they efficiently stained the cell membrane, showing diffuse and dotty localization within the cell at low concentrations (1.0 and 0.1 μM). Specifically, dye TC498 was localized in vesicular structures in both live and fixed cells and could be used as a suitable marker in co-staining studies with other commonly used fluorescent probes.
Τίτλος πηγής δημοσίευσης:	Colorants
Τόμος/Κεφάλαιο:	3
Τεύχος:	1
Θεματική Κατηγορία:	[EL] Οργανική χημεία[EN] Organic chemistry [EL] Φασματοσκοπία[EN] Spectroscopy [EL] Βιοτεχνολογία[EN] Biotechnology [EL] Κυτταρολογία[EN] Cytology
Λέξεις-Κλειδιά:	BODIPYs synthesis DFT calculations cellular biodistribution time-lapse fluorescent imaging confocal microscopy
Χρηματοδότης:	European Regional Development Fund
Χρηματοδοτικό πρόγραμμα:	BIOIMAGING-GR
Αναγνωριστικό χρηματοδοτικού προγράμματος:	5002755
Κάτοχος πνευματικών δικαιωμάτων:	© 2023 by the authors. Licensee MDPI, Basel, Switzerland.
Όροι και προϋποθέσεις δικαιωμάτων:	This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://doi.org/10.3390/colorants3010002
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο