Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Kinetics and mechanism of lipase catalyzed monoacylglycerols synthesis
Δημιουργός/Συγγραφέας:	Itabaiana Jr. I. Gonçalves K.M. Cordeiro Y.M.L. [EL] Ζουμπανιώτη, Μαρία[EN] Zoumpanioti, Maria Leal I.C.R. Miranda L.S.M. De Souza R.O.M.A. [EL] Ξενάκης, Αριστοτέλης[EN] Xenakis, Aristotelis
Εκδότης:	Elsevier
Ημερομηνία:	2013
Γλώσσα:	Αγγλικά
ISSN:	1381-1177
DOI:	10.1016/j.molcatb.2013.06.008
Περίληψη:	Monoacylglycerols are increasingly used in several industrial applications as effective and cheap emulsifiers. In the present work monostearin synthesis has been studied, using lipase as a biocatalyst of the esterification reaction of stearic acid with (R,S)-1,2-O-iso-propylidene glycerol (solketal). The lipase from Candida antarctica (CaL B) was immobilized in AOT/isooctane water in oil microemulsions. Optimization of the reaction conditions have shown that the highest production (80% in 30 min) could be achieved at 40 °C, in microemulsions with relatively low water content (wo = 8). Kinetic studies have shown that the esterification reaction of stearic acid with solketal catalyzed by CaL B occurs via the ordered bi-bi mechanism, in which inhibition by the acid was identified. Moreover, at high fixed solketal concentrations a negative cooperativity is pronounced, which means that binding of the alcohol lowers the affinity of the enzyme for binding of the acid. Values of all kinetic parameters have been determined.
Τίτλος πηγής δημοσίευσης:	Journal of Molecular Catalysis B: Enzymatic
Τόμος/Κεφάλαιο:	96
Σελίδες:	34-39
Θεματική Κατηγορία:	[EL] Χημεία (Γενικά)[EN] Chemistry (General) [EL] Τεχνολογία (Γενικά)[EN] Technology (General)
Λέξεις-Κλειδιά:	Biocatalysis Lipase Microemulsion Monostearin Ordered bi-bi Solketal
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2013 Elsevier B.V.
Σημειώσεις:	Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, CAPES
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο