Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	DNA Damage Response and Redox Status in the Resistance of Multiple Myeloma Cells to Genotoxic Treatment
Δημιουργός/Συγγραφέας:	Malamos, Panagiotis Papanikolaou, Christina Deligianni, Elisavet Mavroeidi, Dimitra Koutoulogenis, Konstantinos Gavriatopoulou, Maria Terpos, Evangelos [EL] Σουλιώτης, Βασίλης Λ.[EN] Souliotis, Vassilis L.
Ημερομηνία:	2025-10-19
Γλώσσα:	Αγγλικά
ISSN:	1422-0067
DOI:	10.3390/ijms262010171
Άλλο:	41155462
Περίληψη:	The DNA Damage Response (DDR) network is an essential machinery for maintaining genomic integrity, with DDR defects being implicated in cancer initiation, progression, and treatment resistance. Moreover, oxidative stress, an imbalance between reactive oxygen species production and antioxidant defense, can significantly impact cell viability, leading to cell death or survival. Herein, we tested the hypothesis that DDR-related signals and redox status measured in multiple myeloma (MM) cell lines correlate with the sensitivity to genotoxic insults. At baseline and following irradiation with Ultraviolet C (UVC; 50 J/m2) or treatment with melphalan (100 μg/mL for 5 min) DDR-related parameters, redox status expressed as GSH/GSSG ratio and apurinic/apyrimidinic sites were evaluated in a panel of eleven human MM cell lines and one healthy B lymphoblastoid cell line. We found that MM cell lines with increased apoptosis rates displayed significantly higher levels of endogenous/baseline DNA damage, reduced GSH/GSSG ratio, augmented apurinic/apyrimidinic lesions, decreased nucleotide excision repair and interstrand crosslinks repair capacities, and highly condensed chromatin structure. Taken together, these findings demonstrate that DDR-related parameters and redox status correlate with the sensitivity of MM cells to DNA-damaging agents, specifically melphalan, and, if further validated, may be exploited as novel sensitive/effective biomarkers.
Τίτλος πηγής δημοσίευσης:	International journal of molecular sciences
Τόμος/Κεφάλαιο:	26
Τεύχος:	20
Θεματική Κατηγορία:	[EL] Μοριακή Βιολογία[EN] Molecular Biology [EL] Κυτταρολογία[EN] Cytology [EL] Νεοπλάσματα. Όγκοι. Ογκολογία (περ. Καρκίνος, κακινογόνες ουσίες)[EN] Neoplasms. Tumors. Oncology (Incl.cancer, carcinogens) [EL] Βιοχημεία[EN] Biochemistry
Λέξεις-Κλειδιά:	DNA damage response (DDR) apoptosis rates apurinic/apyrimidinic sites chromatin condensation interstrand crosslinks (ICL) melphalan multiple myeloma (MM) nucleotide excision repair (NER) oxidative stress resistance to genotoxic treatment
Χρηματοδότης:	European Union
Αναγνωριστικό χρηματοδοτικού προγράμματος:	101097094-ELMUMY
Κάτοχος πνευματικών δικαιωμάτων:	© 2025 by the authors. Licensee MDPI, Basel, Switzerland.
Όροι και προϋποθέσεις δικαιωμάτων:	This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/ licenses/by/4.0/).
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://doi.org/10.3390/ijms262010171
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο