Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	From bread waste to bacterial cellulose nanostructures: Development of a novel rotating disk bioreactor
Δημιουργός/Συγγραφέας:	Pilafidis, Sotirios Vardaxi, Antiopi Kourmentza, Konstantina [EL] Πίσπας, Στέργιος[EN] Pispas, Stergios Dimopoulou, Maria Tsouko, Erminta
Ημερομηνία:	2025-06
Γλώσσα:	Αγγλικά
ISSN:	01418130
DOI:	10.1016/j.ijbiomac.2025.144374
Άλλο:	40393584
Περίληψη:	A novel rotating disk bioreactor was designed and manufactured to produce bacterial cellulose (BC) using Komagataeibacter rhaeticus. Optimal conditions-45 % disk submersion, mechanically etched disks with rotation speed of 20 rpm, spacing of 35 mm and 0.5 vvm air supply- achieved a yield of 1020 mg BC/disk with commercial glucose. Bread waste enzymatic hydrolysates improved BC production by 133.3 %, highlighting the potential of waste valorization in sustainable biopolymer production. BC was further modified into nanostructures (BNCs) using H2SO4 (BNC1), H2SO4- HCl (BNC2), and cellulases (BNC3). FTIR spectra of BC and BNCs revealed typical cellulose vibration-bands while dynamic light scattering showed bimodal or trimodal size distributions (hydrodynamic radiuses of 60-2969 nm). TEM imaging of BNC1 and BNC2 demonstrated rodlike/needlelike nanostructure with widths of 33.1 ± 18.2 nm and 24.8 ± 14.2 nm respectively. BNC3 presented buddle of flat ribbons (width of 56.4 ± 26.3 nm). The maximum degradation temperature of BC (295 °C) decreased after its ex-situ modification (271-294 °C). The enhanced production and tailored structural modifications of BC highlight its potential for diverse applications in materials science. This transformative approach that integrates bread waste valorization and innovative bioreactor design paves the way for high-value advancements in biotechnology and environmental sustainability.
Τίτλος πηγής δημοσίευσης:	International journal of biological macromolecules
Τόμος/Κεφάλαιο:	314
Θεματική Κατηγορία:	[EL] Βιοτεχνολογία[EN] Biotechnology [EL] Βιοχημεία[EN] Biochemistry [EL] Μικροβιολογία[EN] Microbiology [EL] Αναλυτική χημεία[EN] Analytical chemistry
Λέξεις-Κλειδιά:	Bacterial nanocellulose Bioprocess Bioreactor design Renewable resources Tunable properties
Χρηματοδότης:	Hellenic Foundation for Research and Innovation (H.F.R.I.)
EU Grant:	3rd Call for H.F.R.I. Research Projects to support Post-Doctoral Researchers
EU Grant identifier:	7664
Κάτοχος πνευματικών δικαιωμάτων:	/© 2025 The Authors. Published by Elsevier B.V.
Όροι και προϋποθέσεις δικαιωμάτων:	This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://doi.org/10.1016/j.ijbiomac.2025.144374
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο