Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Amphiphilic Chlorin-β-cyclodextrin Conjugates in Photo-Triggered Drug Delivery: The Role of Aggregation
Δημιουργός/Συγγραφέας:	Panagiotakis, Stylianos Mavroidi, Barbara Athanasopoulos, Alexandros [EL] Χαραλαμπίδης, Γεώργιος[EN] Charalambidis, Georgios Coutsolelos, Athanassios G Pelecanou, Maria Yannakopoulou, Konstantina
Χορηγός :	General Secretariat for Research and Technology (Greece)
Ημερομηνία:	2024-06
Γλώσσα:	Αγγλικά
ISSN:	2192-6506 2192-6506
DOI:	10.1002/cplu.202300743
Άλλο:	38345604
Περίληψη:	Conjugates of chlorins with β-cyclodextrin connected either directly or via a flexible linker were prepared. In aqueous medium these amphiphilic conjugates were photostable, produced singlet oxygen at a rate similar to clinically used temoporfin and formed irregular nanoparticles via aggregation. Successful loading with the chemotherapeutic drug tamoxifen was evidenced in solution by the UV-Vis spectral changes and dynamic light scattering profiles. Incubation of MCF-7 cells with the conjugates revealed intense spotted intracellular fluorescence suggestive of accumulation in endosome/lysosome compartments, and no dark toxicity. Incubation with the tamoxifen-loaded conjugates revealed also practically no dark toxicity. Irradiation of cells incubated with empty conjugates at 640 nm and 4.18 J/cm2 light fluence caused >50 % cell viability reduction. Irradiation following incubation with tamoxifen-loaded conjugates resulted in even higher toxicity (74 %) indicating that the produced reactive oxygen species had triggered tamoxifen release in a photochemical internalization (PCI) mechanism. The chlorin-β-cyclodextrin conjugates displayed less-lasting effects with time, compared to the corresponding porphyrin-β-cyclodextrin conjugates, possibly due to lower tamoxifen loading of their aggregates and/or their less effective lodging in the cell compartments' membranes. The results suggest that further to favorable photophysical properties, other parameters are important for the in vitro effectiveness of the photodynamic systems.
Τίτλος πηγής δημοσίευσης:	ChemPlusChem
Τόμος/Κεφάλαιο:	89
Τεύχος:	6
Θεματική Κατηγορία:	[EL] Φαρμακευτική χημεία[EN] Pharmaceutical chemistry [EL] Φυσική και θεωρητική χημεία[EN] Physical and theoretical chemistry [EL] Εφαρμοσμένη οπτική. Φωτονική[EN] Applied optics. Photonics [EL] Κυτταρολογία[EN] Cytology
Λέξεις-Κλειδιά:	aggregation PCI chlorin conjugates cyclodextrins drug delivery
EU Grant identifier:	T3EPA-0048
Κάτοχος πνευματικών δικαιωμάτων:	© 2024 The Authors. ChemPlusChem published by Wiley-VCH GmbH
Όροι και προϋποθέσεις δικαιωμάτων:	This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://doi.org/10.1002/cplu.202300743
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο