Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Coassembly of a Hybrid Synthetic-Biological Chitosan-g-Poly(N-isopropylacrylamide) Copolymer with DNAs of Different Lengths
Δημιουργός/Συγγραφέας:	Karayianni, Maria Lotos, Elena-Daniela Mihai, Marcela [EL] Πίσπας, Στέργιος[EN] Pispas, Stergios
Ημερομηνία:	2024-11-04
Γλώσσα:	Αγγλικά
ISSN:	2073-4360
DOI:	10.3390/polym16213101
Άλλο:	39518309
Περίληψη:	Natural polysaccharides can serve as carriers of genes owing to their intrinsic biocompatibility, biodegradability, and low toxicity. Additionally, they can be easily chemically modified, e.g., through grafting, leading to hybrid synthetic-biological copolymers with additional functionalities. In this work we report on the electrostatic interaction between a chitosan-g-poly(N-isopropylacrylamide) (Chit-g-PNIPAM) copolymer and DNA macromolecules of different lengths (i.e., 50 and 2000 bp), towards the construction of polyplexes that can serve as potential gene delivery systems. At the basic science level, the work aims to elucidate the effects of DNA length on the structural and physicochemical properties of the thermoresponsive hybrid macromolecular assemblies. The protonated amino groups on the chitosan backbone enable electrostatic binding with the anionic phosphate groups of the DNA molecules, while the PNIPAM side chains are expected to impart thermoresponsive properties to the formed polyplexes. Different amino to phosphate group (N/P) mixing ratios were examined, aiming to produce stable dispersions. The physicochemical properties of the resulting polyplexes were investigated by dynamic and electrophoretic light scattering (DLS and ELS), while their morphology was studied by scanning-transmission electron microscopy (STEM). Moreover, their response to changes in temperature and ionic strength, as well as their stability against biological media, was also examined. Finally, the binding affinity of the copolymer towards DNA was evaluated through fluorescence spectroscopy, using ethidium bromide quenching assays, while infrared spectroscopy was used to investigate the structure of the incorporated DNA chains.
Τίτλος πηγής δημοσίευσης:	Polymers
Τόμος/Κεφάλαιο:	16
Τεύχος:	21
Θεματική Κατηγορία:	[EL] Βιοιατρική μηχανική. Ηλεκτρονική. Ενοργάνιση[EN] Biomedical engineering. Electronics. Instrumentation [EL] Νανοτεχνολογία[EN] Nanotechnology
Λέξεις-Κλειδιά:	DNA chitosan electrostatic interactions gene delivery graft copolymers poly(N-isopropylamide) thermoresponsiveness
EU Grant identifier:	PNRR-III-C9-2022-I8-201
Κάτοχος πνευματικών δικαιωμάτων:	© 2024 by the authors. Licensee MDPI, Basel, Switzerland.
Όροι και προϋποθέσεις δικαιωμάτων:	This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://doi.org/10.3390/polym16213101
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο