Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	pH-response of protein-polysaccharide multilayers adsorbed on a flat gold surface: A surface plasmon resonance study
Δημιουργός/Συγγραφέας:	Katsenou, Nikolitsa Spiliopoulos, Nikolaos Anastassopoulos, Dimitrios L [EL] Παπαγιαννόπουλος, Αριστείδης[EN] Papagiannopoulos, Aristeidis Toprakcioglu, Chris
Ημερομηνία:	2024-09
Γλώσσα:	Αγγλικά
ISSN:	0006-3525 1097-0282
DOI:	10.1002/bip.23609
Άλλο:	38899576
Περίληψη:	Polysaccharide-protein multilayers (PPMLs) consisting of bovine serum albumin (BSA) and chondroitin sulfate (CS) are assembled in acidic solution (pH 4.2) via layer-by-layer deposition method. The formation of PPMLs on gold surface and their responsiveness to pH change from 4.2 to 7 is investigated by Surface Plasmon Resonance Spectroscopy. The buildup of the multilayer at pH 4.2 exhibits non-linear growth while the formation of the first layers is strongly affected by the physicochemical properties of the gold surface. Neutral solution (pH 7) affects the interactions between the biopolymers and results in a partially disassemble (disintegration) of the multilayer film. On one hand, the single pair of layers, BSA-CS and the double pair of layers, (BSA-CS)2, assemblies are stable in neutral pH, a result that will be of interest for biomedical applications. On the other hand, multilayer films consisting of more than four layers that is (BSA-CS)2<n<5, disintegrated down to the 4-layered structure by changing pH to neutral, a fact that renders the (BSA-CS)n assembly useful in the field of drug and protein delivery. The residual mass after the disintegration of the assembly never falls below the mass of four layers. The disintegrated multilayer film can be reconstructed and disassembled repeatedly, simply by cycling the pH value.
Τίτλος πηγής δημοσίευσης:	Biopolymers
Τόμος/Κεφάλαιο:	115
Τεύχος:	5
Θεματική Κατηγορία:	[EL] Βιολογική μηχανική[EN] Bioengineering [EL] Βιοιατρική μηχανική. Ηλεκτρονική. Ενοργάνιση[EN] Biomedical engineering. Electronics. Instrumentation
Λέξεις-Κλειδιά:	layer‐by‐layer multilayer pH‐responsiveness protein‐polysaccharide surface plasmon
Κάτοχος πνευματικών δικαιωμάτων:	© 2024 Wiley Periodicals LLC.
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://doi.org/10.1002/bip.23609
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο