Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Physicochemical and Spectroscopic Characterization of Glycogen and Glycogen Phosphorylase b Complexes
Δημιουργός/Συγγραφέας:	Karakousi, Pandora Karayianni, Maria [EL] Χρυσίνα, Ευαγγελία Δ.[EN] Chrysina, Evangelia D. [EL] Πίσπας, Στέργιος[EN] Pispas, Stergios
Ημερομηνία:	2024
Γλώσσα:	Αγγλικά
ISSN:	2673-4176
DOI:	10.3390/polysaccharides5030017
Περίληψη:	Glycogen is a natural polysaccharide used as an energy storage macromolecule. The role of glycogen metabolism in type 2 diabetes mellitus has been under investigation for several years, along with its implication in cancer and cardiovascular and neurodegenerative diseases. Previous studies using pig liver glycogen with rabbit muscle glycogen phosphorylase (RMGPb), which catalyzes the first step of glycogen degradation to glucose-1-phosphate, showed that the surface of an average glycogen molecule is covered by a total of 20 RMGPb dimeric molecules. In this work, we selected oyster glycogen (Glyc) to investigate its interaction with RMGPb by employing biophysical techniques. Dynamic, static, and electrophoretic light scattering were used to investigate the solution behaviors and structures of both the Glyc molecule itself and the formed complexes between Glyc and GPb at different mixing ratios. It was established that the interaction between oyster Glyc and RMGPb is similar to that previously reported for pig liver glycogen. Moreover, the structure of the complexed GPb was monitored by fluorescence and FTIR spectroscopy.
Τίτλος πηγής δημοσίευσης:	Polysaccharides
Τόμος/Κεφάλαιο:	5
Τεύχος:	3
Θεματική Κατηγορία:	[EL] Βιοχημεία[EN] Biochemistry [EL] Δομική Βιολογία[EN] Structural Biology [EL] Φασματοσκοπία[EN] Spectroscopy
Λέξεις-Κλειδιά:	glycogen biopolymer glycogen phosphorylase b biophysical characterization enzyme–biopolymer complexes
EU Grant:	Human Resources Development, Education and Lifelong Learning, Greece and the European Union INSPIRED
EU Grant identifier:	MIS 5002550
Κάτοχος πνευματικών δικαιωμάτων:	© 2024 by the authors. Licensee MDPI, Basel, Switzerland.
Όροι και προϋποθέσεις δικαιωμάτων:	This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://doi.org/10.3390/polysaccharides5030017
Σημειώσεις:	The implementation of the present work is part of Pandora Karakousi’s doctoral thesis and has been co-financed by Greece and the European Union (European Social Fund-ESF) through the Operational Programme “Human Resources Development, Education and Lifelong Learning” in the context of the act titled “Enhancing Human Resources Research Potential by undertaking a Doctoral Research” Sub-action 2: IKY Scholarship Programme for PhD candidates in the Greek Universities. We also acknowledge the support of this work that was provided by Instruct-hub in NHRF by the project “INSPIRED” (MIS 5002550) under the action “Reinforcement of the Research and Innovation Infrastructure”, funded by the Operational Programme “Competitiveness, Entrepreneurship and Innovation” (NSRF 2014–2020).
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο